Cat. No.: X24-03-YW0143
Biotin/STP PEG reagent, Biotin-PEG4-STP ester, Purity 95%
Synonym: Biotin-PEG4-STP Ester
Properties
Description
Biotin-PEG4-STP ester (4-Sulfo-2,3,5,6-tetrafluorophenyl ester) is an amine-reactive labeling reagent designed for use in aqueous environments. It is commonly employed to modify proteins, antibodies, and other amine-containing biopolymers in 100% aqueous media. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent facilitates water solubility, thereby minimizing aggregation of labeled proteins stored in solution.
Molecular Formula
C27H37F4N3O11S2
Functional Group 1
Biotin
Solubility
DMSO, DMF, Water
Identity
Confirmed by NMR.
Applications
Biotin-PEG4-STP ester finds widespread application in bioconjugation studies, allowing specific attachment of biotin to proteins and peptides under mild reaction conditions in aqueous solutions. The resulting biotinylated molecules are frequently utilized in various downstream applications such as affinity purification, immunoprecipitation, and protein detection assays, including Western blotting and immunofluorescence microscopy. Additionally, the water solubility imparted by the PEG spacer arm enhances the stability and solubility of the labeled biomolecules, making them suitable for long-term storage and downstream biochemical analyses.
For Research Use Only. Not For
Clinical Use.