Description
Biotin-PEG4-STP ester (4-Sulfo-2,3,5,6-tetrafluorophenyl ester) is an amine-reactive labeling reagent designed for use in aqueous environments. It is commonly employed to modify proteins, antibodies, and other amine-containing biopolymers in 100% aqueous media. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent facilitates water solubility, thereby minimizing aggregation of labeled proteins stored in solution.
Applications
Biotin-PEG4-STP ester finds widespread application in bioconjugation studies, allowing specific attachment of biotin to proteins and peptides under mild reaction conditions in aqueous solutions. The resulting biotinylated molecules are frequently utilized in various downstream applications such as affinity purification, immunoprecipitation, and protein detection assays, including Western blotting and immunofluorescence microscopy. Additionally, the water solubility imparted by the PEG spacer arm enhances the stability and solubility of the labeled biomolecules, making them suitable for long-term storage and downstream biochemical analyses.