Description
Biotin-PEG4-TFP ester is a biotin labeling reagent specifically designed to react with primary amino (-NH2) groups, forming stable and irreversible amide bonds. The inclusion of a hydrophilic PEG spacer arm enhances the water solubility of the resulting biotinylated molecule.
Applications
Biotin-PEG4-TFP ester is widely utilized for the site-specific biotinylation of proteins, peptides, and other amino-containing biomolecules. The conjugated biotin moiety enables subsequent detection, purification, or immobilization of the labeled biomolecules using streptavidin-based techniques such as Western blotting, ELISA, or immunoprecipitation.