DBCO/NHS PEG reagent, DBCO-NHCO-PEG5-NHS ester, Purity 98%

Synonym: 1378531-80-6; DBCO-NHCO-PEG5-NHS ester; DBCO-NH-PEG4-CH₂CH₂COONHS ester; DBCO-PEG5-NHSester; DBCO-PEG(5)-NHS

CAS Number: 1378531-80-6
PubChem CID: 86580458

Size

25 mg; 100 mg; 500 mg

Price

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Datasheet

Datasheet

MSDS

MSDS

Properties

Description

DBCO-NHCO-PEG5-NHS ester is a versatile PEG linker featuring an NHS ester group for specific and efficient reaction with primary amines under neutral or slightly basic conditions. This reagent enables the site-selective modification of primary amine-containing molecules, such as lysine residues in proteins or aminosilane-coated surfaces, via- covalent bond formation. The hydrophilic PEG spacer arm not only enhances water solubility but also provides a flexible linkage that minimizes steric hindrance during conjugation reactions, ensuring optimal bioconjugation efficiency.

Molecular Weight

693.8

Molecular Formula

C₃₆H₄₃N₃O₁₁

Functional Group 1

DBCO

Functional Group 2

NHS

Functional Group 3

None

Reactive Group 1

Thiol

Form

Oil or amorphous solid

Purity

98%

Solubility

DMSO, DMF, DCM

Identity

Confirmed by NMR.

Applications

DBCO-NHCO-PEG5-NHS ester is widely employed in bioconjugation chemistry for the functionalization of proteins, peptides, antibodies, and other primary amine-bearing biomolecules. Its NHS ester functionality enables efficient coupling with primary amines under mild conditions, allowing for the introduction of DBCO moieties onto specific sites of interest. This facilitates subsequent conjugation reactions with azide-functionalized molecules via- copper-free Click Chemistry, providing a bioorthogonal and chemoselective approach for biomolecule labeling and modification.

Storage

Store at -20°C

For Research Use Only. Not For Clinical Use.